Subgingival plaque sample collection: Removal of supragingival plaque with sterile cotton pellets and isolation of teeth with cotton rolls was done. Subgingival plaque was then collected with sterile paper points (Meta Biomed ® Co. Ltd, Cheongju, South Korea) inserted into the gingival sulcus of Ramfjord’s index teeth for 15 seconds(7-9).
subgingival plaque can be successfully cultured, but the deeper layer may be missed [10]. In comparison, curettes can collect subgingival plaque from the entire periodontal pocket, but the active periodontal pathogens in the outer layer of the plaque can be underestimated with this method [11].
Candida albicans was the most frequently isolated species followed by Candida krusei and Candida tropicalis. subgingival plaque. Technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. However, it seems necessary to assess variations in subgingival plaque caused by orthodontic appliances. The aim of this study was to investigate changes in subgingival plaque: ( sŭb-jinji-văl plak ) Plaque on teeth below the gingival margin in gingival crevice and periodontal pockets.
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Background: The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva Materials and Methods: A total of four hundred subgingival plaque samples which were collected from two hundred periodontitis patients (chronic periodontitis First, a crevicular fluid (CF) sample was collected using a washing method, after which a subgingival plaque sample was taken with a curette. Bacteria were. actinomycetemcomitans from subgingival plaque of patients with different forms of periodontitis was National Collection of Type Cultures (London) and. Materials and methods: Subgingival plaque samples were collected from AgP cases (n=73) and healthy controls (n =71). Bacterial DNA was extracted and 2 Jan 2018 At each time point, sample collection and periodontal examinations were performed before any orthodontic treatment.
Sodium bicarbonate powder for supragingival removal of plaque and surface coatings.Made in GermanyRaspberry FlavorA gentle treatment with grain size 40
Section of Periodontology and Division of Stomatology, School of Dental and Oral Surgery, Columbia University, New York. Search for more papers by this author. Albert Salkind D.D.S. 1994-08-01 Subgingival plaque samples were collected and pooled from 4 non-adjacent proximal sites demonstrating at least 6 mm of attachment loss and 5 mm of probe depths.
The frequencies of occurrence of these three oral treponemes in subgingival plaque samples were as follows: T. denticola, 68.0%; T. vincentii, 36.0%; and T. medium, 48.0%. In addition, the number of T. denticola, T. vincentii, and T. medium cells in plaque samples detected by real-time PCR ranged from 3 to 15,184, 1 to 447, and 1 to
However, it seems necessary to assess variations in subgingival plaque caused by orthodontic appliances. The aim of this study was to investigate changes in subgingival plaque: ( sŭb-jinji-văl plak ) Plaque on teeth below the gingival margin in gingival crevice and periodontal pockets. Saliva is the source of mineralization for supragingival calculus, whereas the serum transudate called gingival crevicular fluid furnishes the minerals for subgingival calculus.
1975). Subgingival plaque was frequently characterized by a zone of gram negative and/or motile species located adjacent to the epithelial lining of the pocket while gram positive
Subgingival plaque sample collection. For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently. Clinical examination of periodontal parameters and collection of saliva and pooled subgingival plaque samples from mesial-buccal sites of 4 first molars were performed before initial therapy and 2, 4 and 6 months respectively after mechanical therapy, and saliva samples were also collected 2 weeks after therapy.
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Prevalence of Candida species in subgingival plaque samples was quantified as 73.3% in Group 1, 66.6% in Group 2 and 60% in Group 3 and no statistically significant differences were observed between groups. Candida albicans was the most frequently isolated species followed by Candida krusei and Candida tropicalis. subgingival plaque.
1975). Subgingival plaque was frequently characterized by a zone of gram negative and/or motile species located adjacent to the epithelial lining of the pocket while gram positive
Subgingival plaque sample collection. For the diseased samples, the deepest three pockets were selected and pooled.
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In this report over 400 subgingival plaque samples taken from over 110 patients were examined Clinical and bacteriological data were collected from over 110.
1. Sample Collection.